时 间：
地 点：博学报告厅
主办单位：海峡联合研究院园艺中心
题 目：Quantitative and functional phosphpproeomics and multiple
Ethylene signaling in Arabidopsis
报 告 人：李凝教授
Quantitative and functional proteomics and post-translational modification (PTM) proteomics have emerged as powerful Omics approaches in studying cellular events in various model organisms. In this seminar, I intend to show several examples on how to apply quantitative and functional PTM proteomics (SILIA, OxNSIL and AQUIP) in investigation of cell signaling in the model plant Arabidopsis and its potential impact in the plant cell biology research in general.
To elucidate the molecular mechanism underlying the time-dependent and dual-and-opposing (DOE) effect of a plant hormone ethylene on a number of plant responses, several well-known Arabidopsis ethylene response mutants, such as ctr1-1, rcn1-1, ein2-5 and eil3eil1 loss-of-function mutant, were selected as target plant materials for the stable isotope metabolic labeling (SIML)-based quantitative phosphoproteomics research. Our quantitative PTM proteomics results clearly revealed that there exist multiple phosphor-relay-mediated ethylene signaling pathways in Arabidopsis, which are EIN2- and EIN3EIL1-independent. This SIML-based quantitative PTM proteomics was able to identify rapidly phosphorylated proteins in response to 1-minute of ethylene treatment from Arabidopsis plants. Reverse genetic and transgenic plant approaches in combination with cell biology studies validated the important biological functions of these candidate phosphoproteins in ethylene-mediated cellular events. These successful research results suggest that the functional PTM proteomic approach is quantitative, repeatable, accurate and versatile in addressing the important biological questions in life sciences.