G7 nations are meeting in Italy to try to hammer out a unified approach to the Syria conflict, in the wake of last week's suspected chemical attack.
Foreign ministers will seek to pressure Russia to distance itself from Syrian President Bashar al-Assad.
UK Foreign Secretary Boris Johnson said possible sanctions against Russian military officials would be discussed.
Allies will also be seeking clarity from the US on its Syria policy, after some apparently mixed messages.
US Secretary of State Rex Tillerson has strongly criticised Russia for failing to prevent Syria from carrying out the chemical attack on the rebel-held town of Khan Sheikhoun last Wednesday that left 89 people dead.
But also said there had been "no change to our military posture" in Syria following a retaliatory US strike against a Syrian airbase, and that Washington's "first priority" in Syria was to defeat so-called Islamic State (IS).
Ahead of the meeting in the city of Lucca, in Tuscany, on Monday, Mr Tillerson took part in an international wreath-laying ceremony to remember those killed in a Nazi massacre of villagers at Sant'Anna di Stazzema in 1944.
He drew a parallel to last week's chemical attack, saying: "We rededicate ourselves to holding to account any and all who commit crimes against the innocents anywhere in the world."
What are the options for pressuring Russia?
Boris Johnson said the message from the meeting should be clear - that Russian President Vladimir Putin must be made to abandon his support for Mr Assad.
"He's toxifying the reputation of Russia by his continual association with a guy who has flagrantly poisoned his own people," Mr Johnson said.
He said the meeting would be "discussing the possibility of further sanctionscertainly on some of the Syrian military figures and indeed on some of the Russian military figures who have been involved in co-ordinating the Syrian military efforts".
Decoding Russia's response to Johnson's cancelled trip
Russia is already under a raft of sanctions imposed by the US and EU in response to the annexation of Crimea and the crisis in eastern Ukraine. These target Russian individuals and businesses, and key sectors of the Russian economy closely connected to the ruling elite.
These would be the first sanctions against Russian figures over Syria if they were to be adopted, but it is far from clear they will be.
Mr Tillerson will want to go from the G7 talks to Moscow on Tuesday to confront the Russians with a unified set of demands.
However, Reuters news agency quoted one senior European diplomat as saying that the US was "navigating aimlessly in the dark" in the search for a transfer of power in Syria.
The G7 groups Canada, France, Germany, Italy, Japan, the UK and the US, with the European Union also represented.